Composite

Part:BBa_K5246046:Design

Designed by: Edgaras Zaboras   Group: iGEM24_Vilnius-Lithuania   (2024-09-26)


Caulobacter crescentus CB2/CB2A HfsA-HfsB-HfsD-HfsF-HfsC-HfsI Polysaccharide export apparatus


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1740
    Illegal XbaI site found at 5937
    Illegal PstI site found at 4245
    Illegal PstI site found at 5744
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1740
    Illegal PstI site found at 4245
    Illegal PstI site found at 5744
    Illegal NotI site found at 475
    Illegal NotI site found at 5417
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1740
    Illegal BglII site found at 5163
    Illegal BglII site found at 5535
    Illegal BglII site found at 5940
    Illegal BglII site found at 6996
    Illegal BamHI site found at 3305
    Illegal XhoI site found at 6410
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1740
    Illegal XbaI site found at 5937
    Illegal PstI site found at 4245
    Illegal PstI site found at 5744
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1740
    Illegal XbaI site found at 5937
    Illegal PstI site found at 4245
    Illegal PstI site found at 5744
    Illegal NgoMIV site found at 151
    Illegal NgoMIV site found at 343
    Illegal NgoMIV site found at 352
    Illegal NgoMIV site found at 924
    Illegal NgoMIV site found at 1087
    Illegal NgoMIV site found at 2026
    Illegal NgoMIV site found at 2672
    Illegal AgeI site found at 3363
    Illegal AgeI site found at 6190
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NA


Source

BBa_J64997, BBa_K4165062, BBa_K2621038 and BBa_K731721 are parts of pACYC-Duet-1 backbone. All the other parts are from C. crescentus CB2(DSM 4727)/CB2A(DSM 25117) strains.

References